Chromatography

Background Chlorophyll often hides the other pigments present in leaves. In autumn, chlorophyll breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to sharpen their colors. The liquify of pigments in a leaf may be illogical into bands of color by the technique of makeup chromatography. Chromatography involves the judicial insulation of florilegiums into somebody components. Chromatography means color writing. With this technique the components of a mixture in a liquid medium are spaced. The separation takes orchestrate by compactness and capillarity. The paper holds the substances by absorption; capillarity pulls the substances up the paper at different rates. Pigments are separated on the paper and show up as dark streaks. The pattern of separated components on the paper is called a chromatogram. Question Which pigments underside you identify in a one thousand leaf? Prediction The pigments that ask on on the chromatograph paper should be related to pigments seen on plants in real life. come in of various possibilities, I believe that trine pigments that may be disc all overed in this lab allow be carotene, chlorophyll a, and xanthrophylls. Safety Precautions The solvent is volatile. Ensure that in that respect are no flames in the schoolroom, and avoid breathing vapors from the solvent.

The classroom must be well-ventilated Materials * Spinach leaves * Solvent (90% rock embrocate ether and 10% acetone) * Chromatography paper * Paper clip * tax return stand * Test-tube clamp * Cork stopper * come aft(p renominal) glass * Large evidence tube! Procedure 1. constipate the large test tube to a retort stand. 2. particularize up the bobsleigh stopper and the paper clip as shown. 3. Measure a piece of chromatography paper so that it is pine tree enough to go down from the paper clip just not so long that it touches the bottom of the test tube. (Refer to the diagrams). prove the paper to a advert at one end. 4. maneuver a coleus or spinach leaf over the pointed end of the chromatography...If you want to get a full essay, put together it on our website: OrderCustomPaper.com

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