Biodiesel

African Journal of Biotechnology Vol. 6 (18), pp. 2130-2134, 19 syndicate 2007 Available online at http://www.academicjournals.org/AJB ISSN 16845315 © 2007 Academic Journals Full length look for Paper Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose learn capacity Chuan-chao Dai*, Jie Tao, Feng Xie, Yi-jun Dai and Mo Zhao Jiangsu Key Laboratory for Biological kind and Biotechnology, College of Life Science, Nanjing universal University, Nanjing, Jiangsu Province, 210046, China. Accepted 15 August, 2007 This study explored a strategy to diversify agricultural and forestry residues into microbial lipid, which could be kick upstairs transformed into biodiesel. Among the 250 yeast strains screened for xylose assimilating capacity, eight oleaginous yeasts were selected by Sudan Black B test. The lipid content of these 8 strains was determined by soxhlet beginning method. One strain (T216) was effect to produce lipids up to 36.6%, and it was identified as Rhodotorula glutinis. The best zymolysis conditions were obtained as follows: glucose as carbon seed 100 g/L; yeast extract and peptone as nitrogen sources at, respectively, 8 and 3 g/L; initial pH of 5.0; inoculation volume of 5%; temperature at 28oC, shaking repair of one hundred eighty r/min, cultivated for 96 h.

Under these conditions, R. glutinis amass lipids up to 49.25% on a cellular biomass basis and the like lipid productivity reached 14.66 g/L. Experiments with a 5-L bioreactor under the optimal kitchen-gardening conditions showed that R. glutinis accumulated lipids up to 60.69%, resulting in 23.41 g/L in lipid prod! uctivity. More encouraging results were observed for the lipid production with alternative carbon sources. Corn stalk and Populus euramevicana leaves hydrolysate could be used to substitute glucose. Chemical analysis indicated that biodiesel obtained by transesterification feature similar composition to that from ve haveable oil, one of the widely...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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